Cell Number in Haploid, Diploid and Polyploid Mouse Embryos
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منابع مشابه
The chromosomes of turkey embryos during early stages of parthenogenetic development.
In the early stages of parthenogenetic development in turkey eggs, many blastoderms are mosaics of haploid, diploid and polyploid cells. The genome composition of these blastoderms can be identified by C-banding. They may be generally described as either A-Z/2A-ZZ/nA-nZ or A-W/2A-WW/nA-nW and are found in a nearly 1:1 ratio. The blastoderms showing the W body (W+) become lethal within two days ...
متن کاملChromosome analysis of early postimplantation presumptive haploid parthenogenetic mouse embryos.
The chromosome constitution of early postimplantation presumptive haploid parthenogenetic mouse embryos was examined. All the embryos isolated were at the egg-cylinder stage and seven contained dividing cells. In two of the apparently healthy embryos only haploid mitoses were seen, whereas in five others an approximately equal proportion of haploid and diploid mitoses was observed. Out of 52 ce...
متن کاملDevelopment of parthenogenetic and fertilized mouse embryos in the uterus and in extra-uterine sites.
Mouse eggs were activated with hyaluronidase in vitro and subsequently transferred to the oviduct. In the female reproductive tract they formed morulae and blastocysts which died soon after implantation. Haploid blastocysts were transferred beneath the kidney capsule and here some formed disorganized egg-cylinder structures in a week. Morulae and blastocysts from haploid and diploid parthenogen...
متن کاملKaryotype Characterization of In Vivo- and In Vitro-Derived Porcine Parthenogenetic Cell Lines
Mammalian haploid cell lines provide useful tools for both genetic studies and transgenic animal production. To derive porcine haploid cells, three sets of experiments were conducted. First, genomes of blastomeres from 8-cell to 16-cell porcine parthenogenetically activated (PA) embryos were examined by chromosome spread analysis. An intact haploid genome was maintained by 48.15% of blastomeres...
متن کاملEstablishment of pluripotential cell lines from haploid mouse embryos.
Eggs from 129 SvE and (C57BL x CBA)F1 hybrid female mice were activated parthenogenetically following their exposure to a 7% solution of ethanol in PBS. Only the haploid class which developed a single pronucleus following second polar body extrusion was examined further. These eggs were transferred to suitable recipients and 'delayed' blastocysts subsequently recovered. The 'delayed' blastocyst...
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تاریخ انتشار 2005